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RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
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Biologia
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JoVE Journal Biologia
RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

DOI:
10.3791/52510-v

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07:01 min

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April 11, 2015

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, ,
1Department of Psychiatry and Neurosciences, Faculty of Medicine,Université Laval, 2Centre de recherche de l’Institut universitaire en santé mentale de Québec

Capitoli

  • 00:05Titolo
  • 01:25Sample Sectioning
  • 02:28Staining
  • 04:06Laser Capture Microdissection (LCM)
  • 05:14Results: Representatve LCM-isolated RNA Analysis
  • 06:30Conclusion

Summary

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Gene expression analysis of a subset of cells in a specific tissue represents a major challenge. This article describes how to isolate high-quality total RNA from a specific cell population by combining a rapid immunolabeling method with laser capture microdissection.

Tags

Laser Capture MicrodissectionLCMRNA IsolationImmunolabelingCell-specific SubpopulationsDopaminergic CellsRNA IntegrityRNA-sequencingMicroarrayQRT-PCR

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