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Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay
 

Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay

Article DOI: 10.3791/52540-v • 08:13 min • February 20th, 2015
February 20th, 2015 •
1, 1, 2
1Genetics and Genomic Medicine, Institute of Child Health, University College London, 2Molecular Cell Science Research Centre, St. George's University of London

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The zebrafish is a popular tool to model chronic kidney disease (CKD). However, their small size makes it impossible to evaluate renal function using traditional methods. We describe a fluorescent dye kidney clearance assay1 that allows quantitative analysis of zebrafish kidney function in CKD.

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Keywords: Zebrafish Kidney Fluorescent Clearance Assay Nephrons Pronephric Tubules Cilia Kidney Function Chronic Kidney Disease Optical Transparency Renal Function
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