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Lepr<sup> Db</sup> Mausmodell des Typ-2-Diabetes: Pancreatic Islet Isolation und Live-cell 2-Photonen-Imaging von intakten Inseln
JoVE Journal
Medicina
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JoVE Journal Medicina
Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
DOI:

10:09 min

May 11, 2015

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Capitoli

  • 00:05Titolo
  • 01:31Reagent Preparation
  • 03:22Islet Isolation and Islet Culturing
  • 06:50Live Cell Photon Imaging
  • 08:06Results: Pancreatic Islet Yield and Insulin Secretion Using 2-Photon Assay
  • 09:37Conclusion

Summary

Traduzione automatica

We present here a protocol for the isolation of islets from the mouse model of type 2 diabetes, Leprdb and details of a live-cell assay for measurement of insulin secretion from intact islets that utilizes 2 photon microscopy.

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