Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Evaluation of Tumor-infiltrating Leukocyte…
JoVE Journal
Medicine

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Medicine
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
 

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Article DOI: 10.3791/52657-v • 07:49 min • April 13th, 2015
April 13th, 2015 •
1, 3, 2,3, 3, 2
1Division of Oncology, Department of Medicine, Washington University School of Medicine, 2Palo Alto Institute for Research and Education, Veterans Affairs Palo Alto Health Care System, 3Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine

Capitoli

Riepilogo
Automatic Translation

This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.

Tags

Keywords: Tumor-infiltrating Leukocytes Tumor Microenvironment Immune Cells Dendritic Cells Regulatory T Cells Myeloid-derived Suppressor Cells Natural Killer Cells Cytotoxic T Cells Tumor Growth Tumor Suppression Flow Cytometry Subcutaneous Tumor Model B16 Melanoma
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter