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Journal / Bioengineering / Measurement of Maximum Isometric Force Generated…
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Bioengineering

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JoVE Journal Bioengineering
Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers
 

Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers

Article DOI: 10.3791/52695-v • 11:30 min • June 16th, 2015
June 16th, 2015 •
1, 1,2, 2,3, 1,2, 3,4
1Department of Orthopaedic Surgery, University of Michigan Medical School, 2Department of Molecular & Integrative Physiology, University of Michigan Medical School, 3Department of Biomedical Engineering, University of Michigan Medical School, 4Department of Surgery, Section of Plastic Surgery, University of Michigan Medical School

Capitoli

Riepilogo
Automatic Translation

Analysis of the contractile properties of chemically skinned, or permeabilized, skeletal muscle fibers offers a powerful means by which to assess muscle function at the level of the single muscle cell. In this article we outline a valid and reliable technique to prepare and test permeabilized skeletal muscle fibers in vitro.

Tags

Maximum Isometric Force Permeabilized Skeletal Muscle Fibers Contractile Properties Single Muscle Fiber Studies Muscle Function Force Production Genetic Manipulations Injury And Disease Muscle Pathologies Force-measuring Apparatus Cross-sectional Area Normalization
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