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In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration
 

In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma

Article DOI: 10.3791/52731-v • 12:48 min • May 11th, 2015
May 11th, 2015 •
1, 1, 2, 1
1Department of Neurobiology & Anatomy, University of Utah, 2Department of Ophthalmology & Visual Sciences, University of Utah

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Microglia activation and microgliosis are key responses to chronic neurodegeneration. Here, we present methods for in vivo, long-term visualization of retinal CX3CR1-GFP+ microglial cells by confocal ophthalmoscopy, and for threshold and morphometric analyses to identify and quantify their activation. We monitor microglial changes during early stages of age-related glaucoma.

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Retinal Microglial Activation Neurodegeneration Confocal Ophthalmoscopic Imaging Cell Morphometry Mouse Glaucoma CNS-resident Neuroimmune Cells Microglial Morphology Microglial Size Activated State Gene Expression Profiles Microglial Activation Monitoring Non-invasive Imaging Chronic Neurodegeneration Live Imaging Retinal Microglia Confocal Ophthalmoscopy CX3CR1GFP/+ Reporter Mice Cellular Resolution Cell Activation Quantification Cell Density Quantification Somal Area Metric
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