Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Generation of Recombinant Human IgG Monoclonal…
JoVE Journal
Immunology and Infection

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunology and Infection
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
 

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Article DOI: 10.3791/52830-v • 10:32 min • June 5th, 2015
June 5th, 2015 •
*1,2, *1, *1, 1, 1, 1, 1, 1
1School for Mental Health and Neuroscience, Maastricht University, 2Department of Neurosciences, Institut d'Investigació Germans Trias i Pujol
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

Synthesis of human monoclonal antibodies is the first step in studies aimed at unraveling the pathophysiological mechanisms of auto-antibody-mediated immune responses. We have developed a protocol to generate recombinant human immunoglobulin G (IgG) monoclonal antibodies from blood sorted B cells, including B-cell isolation, antibody cloning and in vitro synthesis.

Tags

Keywords: Recombinant Human IgG Monoclonal Antibodies Immortalized B Cells Epstein-Barr Virus Toll-like Receptor 9 Peripheral Blood Mononuclear Cells Antibody Cloning Immunotherapeutics Autoimmunity
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter