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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
 

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Article DOI: 10.3791/3738-v • 14:24 min • April 16th, 2012
April 16th, 2012 •
1, 1, 1, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky

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We describe a method for generating regulatory, memory and naïve T cells from a single human blood donor. Polarized Tregs can be then compared to other subsets in a variety of genetic and functional applications with genetic homogeneity, including a suppression assay also detailed here.

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Induced Regulatory T Cells Primary Human Naive T Cells Memory T Cells Immunosuppressive CD4+ Regulatory T Cells Peripheral Tolerance Immunologic Homeostasis Self Antigens Commensal Antigens Treg Biology Therapeutic Injection Of Tregs Murine Models Colitis Type I Diabetes Rheumatoid Arthritis Graft Versus Host Disease Ex Vivo Expansion Peripheral Blood Mononuclear Cells CD4+ T Cells In Vitro Cell Culture Fluorescence Activated Cell Sorting (FACS) Forkhead Box P3 (FoxP3) Activation-induced Transcription Factor
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