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Bioengineering

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JoVE Journal Bioengineering
Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix
 

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

Article DOI: 10.3791/52948-v • 09:26 min • June 12th, 2015
June 12th, 2015 •
1, 2, 3
1Department of Mechanical and Aerospace Engineering, California State University, Long Beach, 2Ximedica, 3School of Engineering, Brown University

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Cell migration is an important part of human development and life. In order to understand the mechanisms that can alter cell migration, we present a planar gradient diffusion system to investigate chemotaxis in a 3D collagen matrix, which allows one to overcome modern diffusion chamber limitations of existing assays.

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Keywords: Cell Migration Chemotaxis 3D Collagen Matrix Traction Force Microscopy Digital Volume Correlation Diffusion Gradient Chemoattractant
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