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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization
 

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

Article DOI: 10.3791/52960-v • 09:25 min • July 28th, 2015
July 28th, 2015 •
1, 1
1Focal Area Infection Biology, Biozentrum, University of Basel

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We describe the quantification of cytosolic and vacuolar Salmonella typhimurium in bone-marrow derived macrophages using differential digitonin permeabilization.

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Quantification Cytosolic Vacuolar Salmonella Primary Macrophages Differential Permeabilization Intracellular Bacterial Pathogens PCVs Shigella Flexneri Francisella Novicida Salmonella Typhimurium Salmonella-containing Vacuole SCV Integrity Phagosome-protection Assay Detergent Digitonin Cholesterol Binding Plasma Membrane Permeabilization Intracellular Membranes Fluorescent Marker Protein MCherry Primary Antibody Fluorophore
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