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Bioengineering

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JoVE Journal Bioengineering
Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis
 

Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis

Article DOI: 10.3791/53045-v • 10:26 min • October 26th, 2015
October 26th, 2015 •
1,2, 3, 3, 3, 2,4, 2,5, 6
1Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 2CREST, Japan Science and Technology Agency, 3School of Fundamental Science and Engineering, Waseda University, 4Research Institute of Electrical Communication, Tohoku University, 5Graduate School of Biomedical Engineering, Tohoku University, 6Faculty of Science and Engineering, Waseda University

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We describe a protocol for modifying cell affinity of a scaffold surface in aqueous environment. The method takes advantage of titanium dioxide photocatalysis to decompose organic film in the photo-irradiated region. We show that it can be used to create microdomains of scaffolding proteins, both ex situ and in situ.

Tags

Photopatterning Proteins Cells Aqueous Environment TiO2 Photocatalysis Organic Contaminants Ultraviolet Light Novel Protocol Cell Affinity Substrate Surface Thin TiO2 Film Sputter-coated Glass Coverslip Organosilane Monolayer Octadecyltrichlorosilane Cell Adhesion Inhibition Cell Culture Medium Focused UV Light Neuronal Cell Line PC12 Cells Adhered Cells UV-irradiated Area In Situ Surface Modification Extracellular Matrix Protein Collagen Patterning Multiple Cell Types Coculture Systems Manipulate Cells
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