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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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Biologia
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JoVE Journal Biologia
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

DOI:
10.3791/53087-v

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07:50 min

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November 25, 2015

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, ,
1Department of Electrical Engineering,University of Washington, 2Department of Computer Science & Engineering,University of Washington

Capitoli

  • 00:05Titolo
  • 00:32Enzymatic Processing of Plasmids
  • 03:06Fluorescence Measurements for a Chemical Reaction A + B -> B + C
  • 06:13Results: Fluorescence Kinetics Measurements (Plasma-derived vs. Synthesized Gates)
  • 07:24Conclusion

Summary

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This protocol describes a method for deriving DNA strand displacement gates from plasmids and testing them using fluorescence kinetics measurements. Gates can be modularly composed into multi-component systems to approximate the behavior of formal chemical reaction networks (CRN), demonstrating a new use for CRNs as a molecular programming language.

Tags

DNA NanotechnologyPlasmid DNANicked Double-stranded DNA (ndsDNA)Strand Displacement GatesChemical Reaction NetworksMolecular ProgrammingEnzymatic ProcessingFluorescence Kinetics

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