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调查器官脊髓联合培养物中生长多电极阵列功能再生

JoVE Journal
Neuroscienze

This content is Free Access.

JoVE Journal Neuroscienze

Investigating Functional Regeneration in Organotypic Spinal Cord Co-cultures Grown on Multi-electrode Arrays

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调查器官脊髓联合培养物中生长多电极阵列功能再生

DOI:

10.3791/53121-v

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08:25 min

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September 23, 2015

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Martina Heidemann, Jürg Streit, Anne Tscherter

¹Department of Physiology,University of Bern

Capitoli

00:05Titolo
01:25Preparation of Multi-Electrode Arrays
02:39Spinal Cord Tissue Dissection
03:52Mounting Spinal Cord Tissue Slices on Multi-Electrode Arrays
05:09Mechanical Lesions
06:11Electrophysiological Recordings of Spontaneous Activity
06:59Results: Synchronized Activity in Slices is Reduced with Age
07:56Conclusion

Summary

Traduzione automatica

在这里，我们提出了一个协议，基于多电极阵列培养来研究在体外 propriospinal连接官能再生脊髓切片。

Tags

Keywords: Organotypic Spinal Cord Co-cultures Multi-electrode Arrays Functional Regeneration Propriospinal Connections Spinal Cord Injury Adult Higher Vertebrates In Vitro Model Network Activity Synchronized Bursts Immunohistochemical Stainings

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