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Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements
DOI:

10:12 min

November 13, 2015

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Capitoli

  • 00:05Titolo
  • 00:45Isolation of Skeletal Muscle
  • 03:47Isolation of Mitochondria from Skeletal Muscle
  • 07:50Mitochondria Membrane Integrity Assay
  • 08:42Results: Membrane Integrity Measurements of the Isolated Preparation
  • 09:31Conclusion

Summary

Traduzione automatica

Here, we present a modification of a previously reported method that allows for the isolation of high quality and purified mitochondria from smaller quantities of mouse skeletal muscle. This procedure results in highly coupled mitochondria that respire with high function during microplate based respirometirc assays.

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