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En optimalisert metode for å isolere og utvide Invariant Natural Killer T-celler fra mus Spleen
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen
DOI:

09:01 min

October 29, 2015

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Capitoli

  • 00:05Titolo
  • 01:06Enrichment, Detection and Purification of Mouse Invariant Natural Killer T (iNKT) Cells
  • 04:55Mouse iNKT Cell Culture and Expansion
  • 07:13Results: Absolute Number and Cytokine Producing Capacity of In Vitro Expanded iNKT Cells
  • 08:30Conclusion

Summary

Traduzione automatica

Here we present an adapted protocol that can be used to generate a large number of murine invariant natural killer T cells from mouse spleen. The protocol outlines an approach by which splenic iNKT cells can be enriched for, isolated and expanded in vitro using a limited number of animals and reagents.

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