Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / An Optimized Method for Isolating and Expanding…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection
An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen
 

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

Article DOI: 10.3791/53256-v • 09:01 min • October 29th, 2015
October 29th, 2015 •
1,2, 1,2, 1,2
1Department of Rheumatology, Laboratory for Molecular Immunology and Inflammation, Ghent University Hospital, 2VIB Inflammation Research Center, Ghent University

Chapters

Summary
Automatic Translation

Here we present an adapted protocol that can be used to generate a large number of murine invariant natural killer T cells from mouse spleen. The protocol outlines an approach by which splenic iNKT cells can be enriched for, isolated and expanded in vitro using a limited number of animals and reagents.

Tags

Invariant Natural Killer T Cells INKT Cells Mouse Spleen Cell Isolation Cell Expansion CD1d Tetramer FACS In Vitro Culture Recombinant Cytokines TCR Stimulation IL-7
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter