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Analysis of Human Natural Killer Cell Metabolism
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JoVE Journal Immunology and Infection
Analysis of Human Natural Killer Cell Metabolism

Analysis of Human Natural Killer Cell Metabolism

DOI:
10.3791/61466-v

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09:03 min

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June 22, 2020

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, ,
1Cardiovascular Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa,Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health

Chapters

  • 00:04Introduction
  • 00:51NK Cells Isolation from Peripheral Blood
  • 03:33Extracellular Flux Assay
  • 06:40Results: NK Cell Function and Metabolism
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

In this paper, we describe a method to measure glycolysis and mitochondrial respiration in primary human Natural Killer (NK) cells isolated from peripheral blood, at rest or following IL15-induced activation. The protocol described could be easily extended to primary human NK cells activated by other cytokines or soluble stimuli.

Tags

Keywords: Natural Killer CellsNK Cell MetabolismExtracellular Flux AnalyzerGlycolysisRespirationLymphocyte SeparationMononuclear CellsNK Cell IsolationIL-15 Stimulation

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