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Journal / Biology / Detection of Intracellular Gene Expression in…
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Biology

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JoVE Journal Biology
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
 

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Article DOI: 10.3791/53268-v • 08:14 min • November 13th, 2015
November 13th, 2015 •
*1, *1, 1, 1, 1, 2, 2,3, 1,4, 1,4
1Department of Cardiovascular Surgery, German Heart Center Munich, Technische Universität München, 2Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universität München, 3Clinical Cooperation Groups: "Antigen-specific Immunotherapy" and "Immune Monitoring", Helmholtz Center Munich (Neuhererg), Technische Universität München, 4DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance
* These authors contributed equally

Capitoli

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Automatic Translation

This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.

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Keywords: Intracellular Gene Expression Live Cells Fluorescence-labeled Nanoparticles Induced Pluripotent Stem Cells Pluripotency Genes Transcription Factors Cell Nucleus Immunohistochemical Staining Gene-specific Nanoparticles Target MRNA Reporter Strand Endocytosis Flow Cytometry
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