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类器官的模型传染病：人类的文化与小鼠胃组织体与幽门螺杆菌的显微注射
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
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类器官的模型传染病：人类的文化与小鼠胃组织体与幽门螺杆菌的显微注射

DOI:
10.3791/53359-v

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10:30 min

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November 12, 2015

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,
1Hubrecht Institute for Developmental Biology and Stem Cell Research,University Medical Centre Utrecht

Capitoli

  • 00:05Titolo
  • 01:01Isolation and Seeding of Gastric Glands
  • 04:58Passage of Gastric Organoid Cultures
  • 06:55Microinjection of Organoid Cultures with Helicobacter pylori
  • 09:02Results: Organoid Formation and Infection with H. pylori
  • 09:48Conclusion

Summary

Traduzione automatica

Traduzione automatica

Stem cell derived cultures harbor tremendous potential to model infectious diseases. Here, the culture of mouse and human gastric organoids derived from adult stem cells is described. The organoids are microinjected with the gastric pathogen Helicobacter pylori.

Tags

OrganoidsGastric OrganoidsHelicobacter PyloriHost-pathogen InteractionsStem CellsInfectious DiseasesCultureMicroinjectionImaging

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