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منهج في النسب الانسحاب من الخلايا القرنية عن طريق متعدد الألوان مراسل الفلورسنت الفئران
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
DOI:

07:48 min

December 18, 2015

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Capitoli

  • 00:05Titolo
  • 00:42Crossing Transgenic Lines of Interest and Tamoxifen Induction
  • 02:19Combining Corneal Injury with Tamoxifen Induction
  • 03:18Preparing Tissue for Imaging
  • 05:19Confocal Microscopy and Image Analysis
  • 06:04Results: Corneal Lineage Tracing under Homeostasis and Injury
  • 06:44Conclusion

Summary

Traduzione automatica

In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of interest.

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