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बहु रंग फ्लोरोसेंट रिपोर्टर चूहों का उपयोग कॉर्नियल प्रकोष्ठों के वंश पता लगाने के लिए एक विधि
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
DOI:

07:48 min

December 18, 2015

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Capitoli

  • 00:05Titolo
  • 00:42Crossing Transgenic Lines of Interest and Tamoxifen Induction
  • 02:19Combining Corneal Injury with Tamoxifen Induction
  • 03:18Preparing Tissue for Imaging
  • 05:19Confocal Microscopy and Image Analysis
  • 06:04Results: Corneal Lineage Tracing under Homeostasis and Injury
  • 06:44Conclusion

Summary

Traduzione automatica

In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of interest.

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