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Immunology and Infection

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JoVE Journal Immunology and Infection
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
 

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Article DOI: 10.3791/53374-v • 08:09 min • November 16th, 2015
November 16th, 2015 •
1, 2, 2, 1, 1, 1
1Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, 2Department of Surgical Sciences, Otolaryngology and Head & Neck Surgery, Akademiska sjukhuset

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Automatic Translation

Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.

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Keywords: Palatine Tonsils B Lymphocytes T Lymphocytes Immune System Pathogen Immune Response Cell Isolation Antibody-dependent Affinity Adenovirus
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