/
/
تحسين توليد المستحثة العضلية باستخدام تعبيد متعدد المقارين تعرب عن النسبة المثلى من Gata4، Mef2c وTbx5
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:57Generation of Neonatal Mouse Cardiac Fibroblasts via Explant Culture
- 03:20Generation of Neonatal Mouse Cardiac Fibroblasts via Enzymatic Digestion
- 05:02Isolation of Thy1.2+ Fibroblasts by Magnetic-activated Cell Sorting (MACS)
- 07:17Retroviral Mediated Reprogramming of Isolated Cardiac Fibroblasts
- 08:52Results: Retroviral Reprogramming of Cardiac Fibroblasts to Induced Cardiac Myocytes
- 09:38Conclusion
We describe here a protocol for the generation of iCMs using retrovirus-mediated delivery of Gata4, Tbx5 and Mef2c in a polycistronic construct. This protocol yields a relatively homogeneous population of reprogrammed cells with improved efficiency and quality and is valuable for future studies of iCM reprogramming.
