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Schnelle enzymatische Prozessierung von Proteinen für MS-Detektion mit einem Durchfluss-Mikroreaktor
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Capitoli
- 00:05Titolo
- 00:41Preparation of the Capillary Microreactor
- 03:07Experimental Setup
- 05:03Sample Loading, Proteolytic Digestion, and Elution for MS Analysis
- 07:05Data Processing
- 07:50Results: Mass Spectrometry Protein Detection Using a Flow-through Microreactor
- 08:11Conclusion
A quick protocol for proteolytic digestion with an in-house built flow-through tryptic microreactor coupled to an electrospray ionization (ESI) mass spectrometer is presented. The fabrication of the microreactor, the experimental setup and the data acquisition process are described.
