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Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
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Biologia
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JoVE Journal Biologia
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

DOI:
10.3791/53586-v

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09:16 min

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October 11, 2015

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,
1Department of Physiology and Pharmacology,College of Veterinary Medicine, University of Georgia

Capitoli

  • 00:05Titolo
  • 00:54Collecting Mouse Oocytes
  • 02:08Oocyte Microinjection
  • 04:42Culturing and Staining the Injected Oocytes
  • 07:52Results: Disruption of Pericentrin Expression in Oocytes during Meiotic Division
  • 08:37Conclusion

Summary

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Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes.

Tags

Meiotic SpindleSiRNAChromosome SegregationAneuploidyMicrotubule Spindle ApparatusMTOCMouse OocytesImmunofluorescence Analysis

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