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El seguimiento de las células en el pez cebra transgénico GFP-Uso del sistema de fotoconvertible PSmOrange
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
DOI:

07:59 min

February 05, 2016

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Capitoli

  • 00:05Titolo
  • 00:42PSmOrange Photoconversion
  • 04:16Analyzing the Fate of Photoconverted PSmOrange Protein Expressing Cells
  • 06:23Results: Cell Tracking in the Zebrafish Pineal Complex Using PSmOrange
  • 07:31Conclusion

Summary

Traduzione automatica

We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.

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