Rivista
/
/
De c-FOS Protein Immunohistologische Detection: Een handig hulpmiddel als een marker van Central Pathways betrokken bij specifieke fysiologische reacties<em> In Vivo</em> en<em> Ex Vivo</em
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
DOI:

05:44 min

April 25, 2016

, , , , , ,

Capitoli

  • 00:05Titolo
  • 00:44Day 1 Endogenous Peroxidase Activity Destruction, Non-specific Binding Site Blockade and Incubation with the Primary Antibody
  • 01:44Day 3 Incubation with Secondary Antibody and Development of a Color Reaction
  • 03:21Sample Mounting
  • 04:16Results: c-FOS-positive Neurons on Sections of the Medulla Oblongata in Control Mice and Mice Submitted to Hypoxia In Vivo
  • 05:17Conclusion

Summary

Traduzione automatica

Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.

Video correlati

Read Article