Rivista
/
Bioingegneria
/
Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
JoVE Journal
Bioingegneria
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Bioingegneria
Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

DOI:
10.3791/53642-v

•

09:14 min

•

August 22, 2016

•

, , ,
1Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore, 2Epithelial Biology Laboratory,Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering,National University of Singapore

Capitoli

  • 00:05Titolo
  • 01:16Macromolecular Crowding in 2D Skin Cell Cultures
  • 02:06Making and Using a Cell-derived Matrix
  • 03:393D Organotypic Skin Co-culture
  • 06:24Results: Macromolecular Crowding of Fibroblasts and Keratinocytes
  • 08:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present a protocol to obtain cell-derived matrices rich in extracellular matrix proteins, using macromolecular crowders (MMC). In addition, we present a protocol which incorporates MMC in 3D organotypic skin co-culture generation, which reduces culture time while maintaining maturity of construct.

Tags

Macromolecular CrowdingExtracellular Matrix Deposition2D And 3D Skin ModelsCell-derived MatricesOrganotypic Skin ConstructsWound TherapiesSkin EquivalentsPrimary FibroblastsPrimary KeratinocytesCo-cultureDecellularizationSodium DeoxycholateCell-derived Matrix

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/53642?article_type=v

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition
check_url/it/53642?article_type=v

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
check_url/it/53642?article_type=v

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology
check_url/it/53642?article_type=v

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
check_url/it/53642?article_type=v

2D and 3D Matrices to Study Linear Invadosome Formation and Activity
check_url/it/53642?article_type=v

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization
check_url/it/53642?article_type=v

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
check_url/it/53642?article_type=v

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
check_url/it/53642?article_type=v

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
check_url/it/53642?article_type=v

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

Read Article