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Capitoli
- 00:05Titolo
- 01:01Infection of A549 Cells
- 03:36Preparation of Whole Cell Extracts
- 05:07Resolution of Whole Cell Extracts by High Resolution SDS-PAGE
- 06:20Analysis of IRF3 Dimerization by Native-PAGE
- 07:37Immunoblot Analysis of IRF3 Species
- 09:23Results: Detection Distinct Phosphorylated IRF3 Species
- 10:56Conclusion
Here we describe a procedure allowing a detailed analysis of the phosphorylation-dependent activation of the IRF3 transcription factor. This is achieved through the combination of a high resolution SDS-PAGE and a native-PAGE coupled to immunoblots using multiple phosphospecific antibodies.
