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Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
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JoVE Journal Chimica
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

DOI:
10.3791/53747-v

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08:42 min

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February 19, 2016

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, ,
1Department of Biology,Virginia Commonwealth University, 2Center for Human Disease Modeling,Duke University Medical Center

Capitoli

  • 00:05Titolo
  • 00:33Embryo Fixation
  • 01:26Immunostaining Whole Embryos
  • 03:47Confocal Imaging and Processing
  • 06:37Results: Immunofluorescence of Phospho-epitopes in Whole Mount Zebrafish Ciliated Organs
  • 08:11Conclusion

Summary

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Techniques are described to immunostain phospho-epitopes in whole zebrafish embryos and then conduct two-color fluorescent confocal localization in cellular structures as small as primary cilia. The techniques for fixing and imaging can define the location and kinetics of the appearance or activation of specific proteins.

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Keywords: ImmunostainingPhospho-epitopesCiliated OrgansWhole MountZebrafish EmbryosCalcium-dependent SignalingImmunolocalizationPhosphorylated ProteinsAcetylated TubulinAnti-phospho CaMKII AntibodyFluorescent Secondary AntibodyBiologia dello sviluppoCell Biology

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