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Developmental Biology

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JoVE Journal Developmental Biology
Feeder-fri Utledning av Melanocytter fra Menneskelig Pluripotent stamceller
 
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Feeder-fri Utledning av Melanocytter fra Menneskelig Pluripotent stamceller

Article DOI: 10.3791/53806-v • 12:21 min • March 3rd, 2016
March 3rd, 2016 •
1,2, 3, 1
1The Center for Stem Cell Biology, Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, 2Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School, Sloan-Kettering Institute for Cancer Research, 3Thermo Fisher Scientific

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Dette arbeidet beskriver en in vitro differensiering protokollen til å produsere pigmenterte, modne melanocytter fra menneskelige pluripotente stamceller via en neural crest og melanoblast mellomstadium ved hjelp av en mater-fri, 25 dagers protokollen.

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Developmental Biology melanocytter pigmentering melanoblast embryonale stamceller (ESCs) menneske Pluripotent stamceller (hPSCs) Induced Pluripotent stamceller (iPSCs) Neural Crest in vitro differensiering sykdom modellering differensiering protokollen menneskelige embryonale stamceller
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