Rivista
/
/
In vivo אלקליין השביט Assay ואנזים-modified אלקליין השביט Assay עבור מדידת Breaks Strand דנ"א נזק חמצוני דנ"א עכברוש הכבד
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver
DOI:

10:38 min

May 04, 2016

, , , ,

Capitoli

  • 00:05Titolo
  • 01:13Preparation of Single Suspensions from Rat Liver
  • 02:54Preparation and Enzyme Treatment of Comet Slides
  • 04:44Unwinding Electrophoresis
  • 06:37DNA Staining, Comet Visualization and Analysis
  • 07:59Results: Measuring DNA Strand Breaks and Oxidative DNA Damage Using the Alkaline Comet Assay
  • 09:27Conclusion

Summary

Traduzione automatica

The alkaline comet assay measures DNA strand breaks in eukaryotic cells. By adding an Endonuclease III or human 8-oxoguanine-DNA-N-glycosylase digestion step, the assay can efficiently detect oxidative DNA damage. We describe methods for using these assays to detect DNA damage in rat liver.

Video correlati

Read Article