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Immunology and Infection

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JoVE Journal Immunology and Infection
The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
 

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

Article DOI: 10.3791/53839-v • 07:27 min • April 18th, 2016
April 18th, 2016 •
1, 1
1Viral and Rickettsial Diseases Department, Naval Medical Research Center

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We described here a simple loop-mediated isothermal amplification (LAMP) method using lyophilized reagents for the detection of C. burnetii in patient samples.

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Keywords: Lyophilized LAMP Reagents Coxiella Burnetii Q Fever DNA Extraction LAMP Reaction Plasmid DNA Serial Dilutions 2x LAMP Buffer Bst DNA Polymerase Agarose Gel Electrophoresis Reconstitution Buffer
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