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Developmental Biology

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JoVE Journal Developmental Biology
Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos
 

Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining

Article DOI: 10.3791/53883-v • 09:44 min • February 2nd, 2016
February 2nd, 2016 •
1,2, 1,2,3,4
1Department of Entomology, National Taiwan University, 2Institute of Biotechnology, National Taiwan University, 3Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 4Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica

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A protocol of whole-mount immunostaining on aphid embryos is presented, in which critical conditions for decreasing background staining and increasing tissue permeability are addressed. These conditions are specifically developed for effective detection of protein expression in the embryonic tissues of aphids.

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Keywords: Immunostaining Pea Aphid Embryos Tissue Permeability Background Staining Germ Cells Laboratory Strain Host Plants Paraformaldehyde Ovaries PBST
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