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Neuroscience

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JoVE Journal Neuroscience
Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
 

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Article DOI: 10.3791/53976-v • 09:23 min • March 26th, 2016
March 26th, 2016 •
*1, *1, 1, 1, 1
1Wolfson Institute for Biomedical Research, University College London
* These authors contributed equally

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Localizing gene expression to specific cell types can be challenging due to the lack of specific antibodies. Here we describe a protocol for simultaneous triple detection of gene expression by combining double fluorescence RNA in situ hybridization with immunostaining.

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Keywords: Double Fluorescence In Situ Hybridization Immunolabelling Gene Expression Mouse Brain Sections Cell Type Localization RNA Probes FITC DIG Tyramide Alkaline Phosphatase Fast Red Immunostaining Plasmid DNA RNA Polymerase Promoters CDNA Restriction Enzyme Linearization Purification Phenol-chloroform Extraction Ethanol Precipitation
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