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瑞士改进轧制技术肠组织准备免疫组化和免疫分析
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Biologia
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JoVE Journal Biologia
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
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瑞士改进轧制技术肠组织准备免疫组化和免疫分析

DOI:
10.3791/54161-v

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07:42 min

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July 13, 2016

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, , ,
1Department of Medicine,Stony Brook University School of Medicine, 2Department of Physiology & Biophysics,Stony Brook University School of Medicine

Capitoli

  • 00:05Titolo
  • 00:48Colon and Small Intestine Preparation
  • 04:38Swiss Rolling
  • 05:55Results: Stained Swiss Roll Intestinal Preparations
  • 07:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

准确识别，并沿着肠道粘膜内层的上皮细胞的位置是必要的，以定义不同的细胞谱系。肠组织的正常成像识别的蛋白表达模式与最大分辨率是至关重要的。本研究旨在描绘处理小鼠肠组织的最佳方法和条件。

Tags

ImmunohistochemicalImmunofluorescentSwiss Rolling TechniqueFixation MethodMouse IntestineAbdominal MusclesColonSmall IntestineCecumModified Bouin’s FixativeGavage Needle

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