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Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

DOI:
10.3791/54190-v

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08:56 min

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July 30, 2016

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, , ,
1Life Sciences Solutions,Thermo Fisher Scientific

Capitoli

  • 00:05Titolo
  • 00:47Induced Pluripotent Stem Cell (iPSC) Generation (d6)
  • 02:14Fibroblast Reprogramming Live Cell Imaging (d19 – 21)
  • 04:16Flow Cytometric Reprogramming Kinetics Measurement
  • 06:35Results: Representative Reprogramming Progression Monitoring
  • 08:10Conclusion

Summary

Traduzione automatica

Traduzione automatica

The protocol presented in this study describes methods for the real-time monitoring of reprogramming progression via the kinetic measurement of positive and negative pluripotent stem cell markers using flow cytometry analysis. The protocol also includes the imaging-based assessment of morphology, and marker or reporter expression during iPSC generation.

Tags

Somatic Cell ReprogrammingInduced Pluripotent Stem CellsIPSCReprogramming KineticsReprogramming EfficiencyCell Surface MarkersReal-time MonitoringFibroblastsTransductionFeeder-independentPhase ContrastFluorescent ImagingAntibody Labeling

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