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דור וזיהוי GM-CSF נגזר מכתשיים דמוי מאקרופאגים דנדריטים תאי עכבר מח עצם
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
DOI:

11:05 min

June 25, 2016

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Capitoli

  • 00:05Titolo
  • 01:11Murine Femur and Tibia Single Bone Marrow Suspesion Preparation
  • 05:55Bone Marrow Cell (BMC) Culture
  • 08:39Results: Representative Bone Marrow Dendritic Cell Culture Analyses
  • 10:13Conclusion

Summary

Traduzione automatica

Bone marrow cells cultured with granulocyte macrophage colony stimulating factor (GM-CSF) generate a heterogeneous culture containing macrophages and dendritic cells (DCs). This method highlights using MHCII and hyaluronan (HA) binding to differentiate macrophages from the DCs in the GM-CSF culture. Macrophages in this culture have many similarities to alveolar macrophages.

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