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Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans
 

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans

Article DOI: 10.3791/54224-v • 10:01 min • August 4th, 2016
August 4th, 2016 •
1, 1,2,3
1Institute of Molecular Biology and Genetics (IMBG), Seoul National University, 2Department of Biological Sciences, Seoul National University, 3Department of Biophysics and Chemical Biology, Seoul National University

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We report a concise procedure of fluorescence in situ hybridization (FISH) in the gonad and embryos of Caenorhabditis elegans for observing and quantifying repetitive sequences. We successfully observed and quantified two different repetitive sequences, telomere repeats and template of alternative lengthening of telomeres (TALT).

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Telomere Repetitive Sequences Fluorescence In Situ Hybridization FISH PNA Probes Caenorhabditis Elegans Telomere Analysis Tumor Regenesis Alternative Lengthening Of Telomeres Cellular Senescence Visualized Telomeres Quantified Telomeres Liquid Media Wash Worms M9 Buffer Centrifugation Bleaching Solution Incubation Time Dissection Microscope
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