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Immunology and Infection

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JoVE Journal Immunology and Infection
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
 

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Article DOI: 10.3791/54263-v • 15:29 min • August 5th, 2016
August 5th, 2016 •
*1, *1, *1,4, *1,3, 2, 2, 1
1Focal Area Infection Biology, Biozentrum, University of Basel, 2Centre d’Immunologie de Marseille-Luminy, Université de la Méditérannée UM2, INSERM U1104 CNRS UM7280, 3Departmento de Microbiologìa and Instituto de Salud Tropical, Universidad de Navarra, 4BioDataAnalysis GmbH
* These authors contributed equally

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Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.

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Microscopy-based Assays High-throughput Screening Host Factors Brucella Infection HeLa Cells Host-pathogen Interaction Semi-automated Workflow Image Analysis Robots Efficient High-throughputs SiRNA Screens Screening Plates Transfection Medium Transfection Reagent Complex Formation HeLa Cell Culture
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