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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes
 

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

Article DOI: 10.3791/54293-v • 08:21 min • July 28th, 2016
July 28th, 2016 •
1, 2, 1
1Department of Microbiology and Immunology, University of Michigan Medical School, 2Department of Biophysics, University of Michigan

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We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.

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HIV-1 Gag Binding Giant Unilamellar Vesicle (GUV) Membranes Bio Assembly Lipid Chains Membrane Binding Virostructural Proteins Visual Detection Post Mixing Steps Lipid Stocks Heat Block Inorganic Solvents Glass Vials Parafilm Lipid Ratios Chloroform-resistant Device
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