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CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
 

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

Article DOI: 10.3791/54401-v • 08:09 min • August 4th, 2016
August 4th, 2016 •
*1,2, *1, *1, 3, 1, 1
1The School of Medical Sciences, University of New South Wales Australia, 2Neuroscience Research Australia, 3Biomedical Imaging Facility, University of New South Wales Australia
* These authors contributed equally

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This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.

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Keywords: CUBIC Protocol Protein Expression Single Cell Resolution Whole Mount Skin Preparations Epidermal Development Skin Regeneration Skin Disease Hair Follicles Skin Biopsy Skin Clearing CUBIC Clearing Solution
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