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Affinity Étiquetage Détection de Endogène Receptors de Zebrafish Embryons
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
DOI:

08:39 min

August 31, 2016

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Capitoli

  • 00:05Titolo
  • 00:47Preparation of Embryo Protein Extract
  • 03:22Detection of Endogenous Receptor Protein
  • 05:45Results: AFLIP Detection of BG in Zebrafish Embryos
  • 07:08Conclusion

Summary

Traduzione automatica

A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to immunoprecipitation.

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