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Genetics

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JoVE Journal Genetics
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
 

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Article DOI: 10.3791/54424-v • 10:01 min • September 27th, 2016
September 27th, 2016 •
1, 1, 1, 1, 1
1Department of Mechanical Engineering, National Taiwan University

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This work presents a simple and visual method to detect multi-nucleotide polymorphisms on a pneumatic droplet manipulation platform. With the proposed method, the entire experiment, including droplet manipulation and detection of multi-nucleotide polymorphisms, can be performed near 23 °C without the aid of advanced instruments.

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Keywords: Multi-nucleotide Polymorphisms Pneumatic Droplet Manipulation Visual Colorimetric Detection Gold Nanoparticles DNA Detection Genetic Variation Genetic Diseases Probe DNA Sodium Chloride Hydroxyl Amine Chloroauric Acid Nanoparticle Optical Properties DNA Fragment Mismatch
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