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Journal / Genetics / Manipulation of Gene Function in Mexican Cavefish
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Genetics

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JoVE Journal Genetics
Manipulation of Gene Function in Mexican Cavefish
 

Manipulation of Gene Function in Mexican Cavefish

Article DOI: 10.3791/59093-v • 07:01 min • April 22nd, 2019
April 22nd, 2019 •
1,2, 1,2, 1,2, 2,3, 1,2, 2,3
1Department of Biological Sciences, Florida Atlantic University, 2Jupiter Life Science Initiative, Florida Atlantic University, 3Harriet L. Wilkes Honors College, Florida Atlantic University

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We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

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Keywords: Cave Fish Astyanax Mexicanus Gene Function Manipulation Morpholino CRISPR Tol2 Transposase Genetic Tools Developmental Biology Model Organism
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