È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:48Harvesting Adipose Tissue
- 02:13Culturing Rat Adipose-derived Stem Cells (rASCs)
- 03:42Establishing rASC Sheet Cultures
- 04:53The Full-thickness Skin Defect Wound Model Using Transplanted rASC Sheets
- 06:54Results: Wound Healing
- 07:20Conclusion
Adipose-derived stem cells (ASCs) are easily isolated and harvested from the fat of normal rats. ASC sheets can be created using cell-sheet engineering and can be transplanted into Zucker diabetic fatty rats exhibiting full-thickness skin defects with exposed bone and then covered with a bilayer of artificial skin.
