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方法确定HCN1和TRIP8b之间蛋白 - 蛋白相互作用的小分子抑制剂
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
DOI:

10:20 min

November 11, 2016

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Capitoli

  • 00:05Titolo
  • 01:08High Throughput Screen
  • 03:07Confirmation of Hits Using TAMRA-tagged HCN1 Peptide
  • 04:33Dose-response Validation of Bead-based Proximity Assay
  • 07:15Results: Identification of Small Molecule Inhibitors Between HCN1 and TRIPb8 Interactions
  • 09:14Conclusion

Summary

Traduzione automatica

HCN通道和其辅助亚基之间的相互作用已被确定为在抑郁症的治疗靶点。这里,用于识别这种蛋白质 - 蛋白质相互作用的小分子抑制剂荧光基于偏振的方法,提出。

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