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Immunology and Infection

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JoVE Journal Immunology and Infection
The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood
 

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Article DOI: 10.3791/54582-v • 08:26 min • December 14th, 2016
December 14th, 2016 •
1
1Graduate Institute of Pharmacology, College of Medicine, National Taiwan University

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Human peripheral blood is commonly used for the assessment of the humoral immune response. Here, the methods for isolating human B cells from peripheral blood, differentiating human B cells into antibody (Ab)-secreting B cells (ASCs) in culture, and enumerating the total IgM- and IgG-ASCs via an ELISpot assay are described.

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Keywords: ELISpot Antibody-secreting B Cells Blood Humoral Immunity Quantification Differentiation Isolation Vaccine Research Immunology Single Cell Detection Biotinylated Antibody Streptavidin Microbeads Magnetic Separation
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