È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:55Processable PANI Synthesis
- 02:40PANI-probe Mixing and UV Irradiation and Hybridization of PANI-probe
- 04:21Emission Steady State Fluorescence Measurement and Fluorescence Microscopy Measurement of Hybridized Duplex
- 05:45Results: Use of PANI Fluorescence to Detect Nucleic Acid Hybridization
- 06:53Conclusion
Nucleic acids are common analytes for assessing biological systems; however, bias from enzymatic manipulation can cause concern. Here a method is described for label-free detection of nucleic acids using polyaniline. This sensitive, cost-effective sensor technology can distinguish single nucleotide differences between molecules.
