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Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
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JoVE Journal Biochimica
Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria

Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria

DOI:
10.3791/54682-v

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08:51 min

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November 10, 2016

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, ,
1Molecular Microbial Biochemistry Laboratory, Faculty of Science (Applied Bioscience Program),University of Ontario Institute of Technology

Capitoli

  • 00:05Titolo
  • 00:45Triple Response Assay
  • 03:14Pellicle Assay
  • 07:03Triple Response Assay and Pellicle Assay Results
  • 08:02Conclusion

Summary

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The protocols outlined herein facilitate the convenient investigation of bacterial ethylene responses by utilizing 2-chloroethylphosphonic acid (CEPA). Ethylene is produced in situ through the decomposition of CEPA in an aqueous bacterial growth medium, circumventing the requirement for pure ethylene gas.

Tags

EthyleneCEPABacterial ResponsePlant-microbe InteractionsArabidopsisCellulose ProductionPlant ColonizationIn Vitro StudyACCGrowth MediumAgarSeed GerminationHypocotyl Measurement

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